Effect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase

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Abstract

Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate that microwave radiations can be profitably exploited to improve reaction kinetics in solid phase peptide synthesis when both chemical and biocatalytic strategies are used.

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Most cited references 26

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Influence of microwave irradiation on enzymatic properties: applications in enzyme chemistry.

Thierry Besson, Barbara Réjasse, Marie Dominique Legoy … (2007)

Although microwave-assisted reactions are widely applied in various domains of organic chemistry, their use in the area of enzyme chemistry has been rather limited, due to the high temperatures associated with the microwave heating: Because current technology, allows a good control of reaction parameters, several examples of microwave-assisted enzyme chemistry have been reported, using stable and effective biocatalysts (modified enzymes). The purpose of this review is to highlight the applications and studies on the influence of microwave irradiation on enzymatic properties and their application in enzyme chemistry.

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Microwave-assisted solid-phase synthesis of peptoids.

Hernando J. Olivos, Prasanna G Alluri, M Muralidhar Reddy … (2002)

Microwave irradiation reduces the reaction time for the solid-phase synthesis of peptoids. Under these conditions, coupling of each residue requires only 1 min. The purity and yields of peptoids synthesized in this way are as good as or better than those achieved using standard methods. [reaction: see text]

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Application of microwave irradiation to the synthesis of 14-helical beta-peptides.

Justin Murray, Samuel H. Gellman (2005)

[structure: see text] We have evaluated the effects of microwave irradiation on the solid-phase synthesis of beta-peptides. Sequences designed to adopt the 14-helix, especially those containing the structure-promoting residue trans-2-aminocyclohexanecarboxylic acid (ACHC), suffer from poor synthetic efficiency under standard SPPS conditions. A comparison of microwave and conventional heating shows that both provide excellent synthetic results for shorter sequences; however, we identify a clear benefit from microwave irradiation for longer beta-peptides.

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Author and article information

Journal

Journal ID (nlm-ta): Int J Pept

Journal ID (publisher-id): IJPEP

Title: International Journal of Peptides

Publisher: Hindawi Publishing Corporation

ISSN (Print): 1687-9767

ISSN (Electronic): 1687-9775

Publication date (Print): 2009

Publication date (Electronic): 25 March 2009

Volume: 2009

Electronic Location Identifier: 362482

Affiliations

¹Laboratory of Applied and Computational Biocatalysis, Department of Pharmaceutical Sciences, University of Trieste, Piazzale Europa 1, 34127 Trieste, Italy

²School of Chemistry, The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK

³Manchester Interdisciplinary Biocentre (MIB), The University of Manchester, 131 Princess Street, Manchester M1 7DN, UK

Author notes

*Alessandra Basso: basso@ 123456units.it

Recommended by Ayman El-Faham

Article

DOI: 10.1155/2009/362482

PMC ID: 2915653

PubMed ID: 20721296

SO-VID: c60f01ff-f5b4-417e-b93e-ace86e0cba34

License:

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.