Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro

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Abstract

Background

The human Obg-like ATPase 1 (OLA1) protein has been reported to play an important role in cancer cell proliferation. The molecular mechanism underlying OLA1 regulated oral metastasis is still unknown. We investigated in this study the regulatory role of OLA1 playing in oral squamous cell metastasis.

Results

A series of in vitro assays were performed in the cells with RNAi-mediated knockdown or overexpression to expound the regulatory function of OLA1 in oral cancer. We found that the endogenous level of OLA1 in a highly metastatic oral squamous cell line was significantly lower than that in low metastatic oral cells as well as in normal oral cells. Escalated expression of OLA1 resulted in a reduced ability of metastasis in highly metastatic cells, and enhanced its sensitivity to the paclitaxel treatment. Further analysis of the EMT markers showed that Snail, Slug, N-cadherin were up-expressed significantly. Meanwhile, E-cadherin was significantly down-regulated in the oral cancer cells with OLA1-knocked down, suggesting that OLA1 inactivated EMT process. Furthermore, we found that OLA1 suppressed oral squamous cell metastasis by suppressing the activity of a TGFβ/SMAD2/EMT pathway.

Conclusion

Our data suggests that OLA1 may be developed as a potential target for the treatment of oral cancer metastasis.

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Most cited references 26

Record: found
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Epithelial-mesenchymal transitions in development and disease.

Jean Paul Thiery, Hervé Acloque, Ruby Y J Huang … (2009)

The epithelial to mesenchymal transition (EMT) plays crucial roles in the formation of the body plan and in the differentiation of multiple tissues and organs. EMT also contributes to tissue repair, but it can adversely cause organ fibrosis and promote carcinoma progression through a variety of mechanisms. EMT endows cells with migratory and invasive properties, induces stem cell properties, prevents apoptosis and senescence, and contributes to immunosuppression. Thus, the mesenchymal state is associated with the capacity of cells to migrate to distant organs and maintain stemness, allowing their subsequent differentiation into multiple cell types during development and the initiation of metastasis.

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Classification and evolution of P-loop GTPases and related ATPases.

Detlef Leipe, Yuri I. Wolf, Eugene V. Koonin … (2002)

Sequences and available structures were compared for all the widely distributed representatives of the P-loop GTPases and GTPase-related proteins with the aim of constructing an evolutionary classification for this superclass of proteins and reconstructing the principal events in their evolution. The GTPase superclass can be divided into two large classes, each of which has a unique set of sequence and structural signatures (synapomorphies). The first class, designated TRAFAC (after translation factors) includes enzymes involved in translation (initiation, elongation, and release factors), signal transduction (in particular, the extended Ras-like family), cell motility, and intracellular transport. The second class, designated SIMIBI (after signal recognition particle, MinD, and BioD), consists of signal recognition particle (SRP) GTPases, the assemblage of MinD-like ATPases, which are involved in protein localization, chromosome partitioning, and membrane transport, and a group of metabolic enzymes with kinase or related phosphate transferase activity. These two classes together contain over 20 distinct families that are further subdivided into 57 subfamilies (ancient lineages) on the basis of conserved sequence motifs, shared structural features, and domain architectures. Ten subfamilies show a universal phyletic distribution compatible with presence in the last universal common ancestor of the extant life forms (LUCA). These include four translation factors, two OBG-like GTPases, the YawG/YlqF-like GTPases (these two subfamilies also consist of predicted translation factors), the two signal-recognition-associated GTPases, and the MRP subfamily of MinD-like ATPases. The distribution of nucleotide specificity among the proteins of the GTPase superclass indicates that the common ancestor of the entire superclass was a GTPase and that a secondary switch to ATPase activity has occurred on several independent occasions during evolution. The functions of most GTPases that are traceable to LUCA are associated with translation. However, in contrast to other superclasses of P-loop NTPases (RecA-F1/F0, AAA+, helicases, ABC), GTPases do not participate in NTP-dependent nucleic acid unwinding and reorganizing activities. Hence, we hypothesize that the ancestral GTPase was an enzyme with a generic regulatory role in translation, with subsequent diversification resulting in acquisition of diverse functions in transport, protein trafficking, and signaling. In addition to the classification of previously known families of GTPases and related ATPases, we introduce several previously undetected families and describe new functional predictions.

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Tobacco smoking and cancer: a meta-analysis.

Sara Gandini, Edoardo Botteri, Simona Iodice … (2008)

We conducted a systematic meta-analysis of observational studies on cigarette smoking and cancer from 1961 to 2003. The aim was to quantify the risk for 13 cancer sites, recognized to be related to tobacco smoking by the International Agency for Research on Cancer (IARC), and to analyze the risk variation for each site in a systematic manner. We extracted data from 254 reports published between 1961 and 2003 (177 case-control studies, 75 cohorts and 2 nested case-control studies) included in the 2004 IARC Monograph on Tobacco Smoke and Involuntary Smoking. The analyses were carried out on 216 studies with reported estimates for 'current' and/or 'former' smokers. We performed sensitivity analysis, and looked for publication and other types of bias. Lung (RR = 8.96; 95% CI: 6.73-12.11), laryngeal (RR = 6.98; 95% CI: 3.14-15.52) and pharyngeal (RR = 6.76; 95% CI: 2.86-15.98) cancers presented the highest relative risks (RRs) for current smokers, followed by upper digestive tract (RR = 3.57; 95% CI: 2.63-4.84) and oral (RR = 3.43; 95% CI: 2.37-4.94) cancers. As expected, pooled RRs for respiratory cancers were greater than the pooled estimates for other sites. The analysis of heterogeneity showed that study type, gender and adjustment for confounding factors significantly influence the RRs estimates and the reliability of the studies. Copyright 2007 Wiley-Liss, Inc.

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Author and article information

Contributors

Gary Guishan Xiao: gxiao@dlut.edu.cn

Journal

Journal ID (nlm-ta): BMC Mol Cell Biol

Journal ID (iso-abbrev): BMC Mol Cell Biol

Title: BMC Molecular and Cell Biology

Publisher: BioMed Central (London )

ISSN (Electronic): 2661-8850

Publication date (Electronic): 14 September 2020

Publication date PMC-release: 14 September 2020

Publication date Collection: 2020

Volume: 21

Electronic Location Identifier: 65

Affiliations

[1 ]GRID grid.30055.33, ISNI 0000 0000 9247 7930, State Key Laboratory of Fine Chemicals, Department of Pharmaceutical Sciences, School of Chemical Engineering, , Dalian University of Technology, ; Dalian, 116024 China

[2 ]GRID grid.30055.33, ISNI 0000 0000 9247 7930, School of Bioengineering, , Dalian University of Technology, ; Dalian, 116024 China

[3 ]GRID grid.19006.3e, ISNI 0000 0000 9632 6718, School of Dentistry, , University of California Los Angeles, ; Los Angeles, 90095 USA

[4 ]GRID grid.411930.e, ISNI 0000 0004 0456 302X, Functional Genomics and Proteomics Center, , Creighton University Medical Center, ; Omaha, 68131 USA

Article

Publisher ID: 311

DOI: 10.1186/s12860-020-00311-z

PMC ID: 7489017

PubMed ID: 32928102

SO-VID: 8af202bd-f8c4-4c78-9621-cb5c99153cb9

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 11 May 2020

Date accepted : 31 August 2020

Funding

Funded by: FundRef http://dx.doi.org/10.13039/501100001809, National Natural Science Foundation of China;

Award ID: 81770846

Award ID: 81803024

Award Recipient : Gary Guishan Xiao

Funded by: FundRef http://dx.doi.org/10.13039/100002135, Hirshberg Foundation for Pancreatic Cancer Research;

Award ID: GX20171003858

Award ID: GX20191005878

Award Recipient : Gary Guishan Xiao

Funded by: Fundamental Research Funds from the Dalian Universities of Technology

Award ID: DUT17ZD308

Award Recipient : Gary Guishan Xiao

Custom metadata

Keywords: metastasis,ola1,emt,oral squamous cell carcinoma,smad2

Data availability:

Keywords: metastasis, ola1, emt, oral squamous cell carcinoma, smad2

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Obg-like ATPase 1 inhibited oral carcinoma cell metastasis through TGFβ/SMAD2 axis in vitro

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Abstract

Background

Results

Conclusion

Related collections

Inflamed lung in vitro co-culture models

Most cited references 26

Epithelial-mesenchymal transitions in development and disease.

Classification and evolution of P-loop GTPases and related ATPases.

Tobacco smoking and cancer: a meta-analysis.

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Cited by 3

Most referenced authors 1,323