There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
A comprehensive understanding of the cellular functions of the Hsp90 molecular chaperone
has remained elusive. Although Hsp90 is essential, highly abundant under normal conditions,
and further induced by environmental stress, only a limited number of Hsp90 "clients"
have been identified. To define Hsp90 function, a panel of genome-wide chemical-genetic
screens in Saccharomyces cerevisiae were combined with bioinformatic analyses. This
approach identified several unanticipated functions of Hsp90 under normal conditions
and in response to stress. Under normal growth conditions, Hsp90 plays a major role
in various aspects of the secretory pathway and cellular transport; during environmental
stress, Hsp90 is required for the cell cycle, meiosis, and cytokinesis. Importantly,
biochemical and cell biological analyses validated several of these Hsp90-dependent
functions, highlighting the potential of our integrated global approach to uncover
chaperone functions in the cell.