Morphometric analysis of astrocytes in vocal production circuits of common marmoset ( Callithrix jacchus )

Abstract

Astrocytes, the star‐shaped glial cells, are the most abundant non‐neuronal cell population in the central nervous system. They play a key role in modulating activities of neural networks, including those involved in complex motor behaviors. Common marmosets ( Callithrix jacchus), the most vocal non‐human primate (NHP), have been used to study the physiology of vocalization and social vocal production. However, the neural circuitry involved in vocal production is not fully understood. In addition, even less is known about the involvement of astrocytes in this circuit. To understand the role, that astrocytes may play in the complex behavior of vocalization, the initial step may be to study their structural properties in the cortical and subcortical regions that are known to be involved in vocalization. Here, in the common marmoset, we identify all astrocytic subtypes seen in other primate's brains, including intralaminar astrocytes. In addition, we reveal detailed structural characteristics of astrocytes and perform morphometric analysis of astrocytes residing in the cortex and midbrain regions that are associated with vocal production. We found that cortical astrocytes in these regions illustrate a higher level of complexity when compared to those in the midbrain. We hypothesize that this complexity that is expressed in cortical astrocytes may reflect their functions to meet the metabolic/structural needs of these regions.

Abstract

In the common marmoset, detailed morphological analysis of 3D‐reconstructed astrocytes residing in vocal production circuits revealed that, in general, cortical astrocytes are structurally more complex than those residing within midbrain regions. We hypothesize that this morphological complexity of cortical astrocytes reflects their function in providing neuronal support or modulation of vocalization.