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      Efecto hormonal y nutricional del anillado en frutos de diferentes tipos de brotes de cítricos Translated title: Girdling effect on fruitlets hormonal and nutritional content in different Citrus shoot types

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          Abstract

          En el presente trabajo se estudió el efecto del anillado de ramas sobre la abscisión de frutos en diferentes tipos de brotes y su relación con el contenido de ácido abscísico (ABA) y carbohidratos durante la fase de cuajado en árboles adultos de mandarina ‘Clemenules’ (Citrus reticulata Blanco). Además, se cuantificó la cosecha luego de la aplicación de inhibidores de la Fase I (Cyocel, CCC) y II (Paclobutrazol, PBZ) de la síntesis de Giberelinas (GAs). Se observó que ambos inhibidores de la síntesis de GAs, redujeron hasta un 40 % la cosecha total, mientras que en combinación con el anillado los niveles fueron similares al testigo sin tratar. Asimismo el PBZ redujo los niveles de azúcares totales mientras que en combinación con el anillado, a los 30 días del tratamiento, se logró restablecer los niveles del control. En frutitos de brotes con y sin hojas el anillado redujo en más de 60 % el nivel de ABA a los siete días luego del tratamiento, siguiendo además una tendencia similar en ambos tipos de brote. Este descenso en los niveles de ABA fue precedido de una reducción de la tasa de abscisión, la cual se mantuvo en niveles significativamente inferiores para los tipos de brotes estudiados hasta los 60 días después del anillado. Para el caso de los brotes sin hojas la abscisión al final del período de evaluación fue similar al control, mientras que en el caso de brotes con hojas se redujo. Se evidencia por tanto el efecto directo del anillado sobre los niveles de ABA, pudiendo explicar así la reducción de la abscisión y la posible implicancia del anillado en la modificación de la relación GAs/ABA durante la fase de cuajado, independientemente del tipo de brote.

          Translated abstract

          In this paper we studied the effect of branch girdling on fruitlets abscission arising from different shoot types, and its relationship with abscisic acid (ABA) and carbohydrates content in adult trees of ‘Clemenules’ mandarin (Citrus reticulata Blanco). Besides, girdling combined with two gibberellin inhibitors of phases I and II (Cycocel and Paclobutrazol), was evaluated by recording total crop at harvest. Both growth inhibitors reduced crop by 40% whereas in combination with girdling reached the level of control trees. Paralleling this effect, PBZ reduced total carbohydrates content in young fruitlets, whereas combined with girdling, within 30 days of treatment, carbohydrates level were restores to that of control trees. Concomitantly, girdling reduced by more than 60 % ABA content in fruitlets at seven days after treatment, following a similar trend in both types of shoots. This decrease in ABA level was followed by a reduction in the rate of fruitlets abscission, which remained at levels significantly lower for the type of shoots studied until 60 days after treatment. Final fruit set of leafless inflorescences was not affected by girdling, whereas leafy inflorescence reduced abscission at the end of physiological fruit drop. Thus, the present work reveals that girdling can improve fruit set by early modifications of carbohydrates and GAs/ABA relationship regardless of shoot type.

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          ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible Cultivars.

          M Simmons (1987)
          A sprouting-resistant and a sprouting-susceptible wheat cultivar were utilized to examine the role of ABA levels and sensitivity responses in wheat embryonic germination. Endogenous embryonic ABA levels were measured in both cultivars throughout grain maturation utilizing a new and sensitive ABA immunoassay. Embryonic ABA levels of each cultivar were similar with the sprouting-susceptible cultivar having about a 25% lower ABA level than that of the sprouting-resistant cultivar. Larger differences between the cultivars were noted in sensitivity to ABA, as measured by capability of ABA to block embryonic germination. ABA inhibited embryonic germination much more effectively in the sprouting-resistant cultivar.
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            Hormonal regulation of fruitlet abscission induced by carbohydrate shortage in citrus.

            The hormonal signals controlling fruitlet abscission induced by sugar shortage in citrus were identified in Satsuma mandarin, Citrus unshiu (Mak.) Marc, cv. Clausellina and cv. Okitsu. Sugar supply, hormonal responses and fruitlet abscission were manipulated through full, partial or selective leaf removals at anthesis and thereafter. In developing fruitlets, defoliations reduced soluble sugars (up to 98%), but did not induce nitrogen and water deficiencies. Defoliation-induced abscission was preceded by rises (up to 20-fold) in the levels of abscisic acid (ABA) and 1-aminocyclopropane-1-carboxylic acid (ACC) in fruitlets. Applications to defoliated plants showed that ABA increased ACC levels (2-fold) and accelerated fruitlet abscission, whereas norflurazon and 2-aminoethoxyvinyl glycine reduced ACC (up to 65%) and fruitlet abscission (up to 40%). Only the full defoliation treatment reduced endogenous gibberellin A1 (4-fold), whereas exogenous gibberellins had no effect on abscission. The data indicate that fruitlet abscission induced by carbon shortage in citrus is regulated by ABA and ACC originating in the fruits, while gibberellins are apparently implicated in the maintenance of growth. In this system, ABA may act as a sensor of the intensity of the nutrient shortage that modulates the levels of ACC and ethylene, the activator of abscission. This proposal identifies ABA and ACC as components of the self-regulatory mechanism that adjusts fruit load to carbon supply, and offers a physiological basis for the photoassimilate competition-induced abscission occurring under natural conditions.
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              Gibberellins and parthenocarpic ability in developing ovaries of seedless mandarins.

              Satsuma (Citrus unshiu [Mak] Marc.) and Clementine (Citrus reticulata [Hort.] Ex. Tanaka, cv Oroval) are two species of seedless mandarins differing in their tendency to develop parthenocarpic fruits. Satsuma is a male-sterile cultivar that shows a high degree of natural parthenocarpy and a high fruit set. Seedless Clementine varieties are self-incompatible, and in the absence of cross-pollination show a very low ability to set fruit. The gibberellins (GAs) GA53, putative 17-OH-GA(53), GA(44), GA(17), GA(19), GA(20), GA(29), GA(1), 3-epi-GA(1), GA(8), GA(24), GA(9), and GA(4) have been identified from developing fruits of both species by full-scan combined gas chromatography-mass spectrometry. Using selected ion monitoring with [(2)H(2)]- and [(13)C]-labeled internal standards, the levels of GA(53), GA(44), GA(19), GA(20), GA(1), GA(8), GA(4), and GA(9) were determined in developing ovaries at anthesis and 7 days before and after anthesis, from both species. Except for GA8, levels of the 13-hydroxy-GAs were higher in Satsuma than in Clementine, and these differences were more prominent for developing young fruits. At petal fall, Satsuma had, on a nanograms per gram dry weight basis, higher levels of GA(53) (10.4x), GA(44) (13.9x), GA(19) (3.0x), GA(20) (11.2x), and GA(1) (2.0x). By contrast, levels of GA(8) were always higher in Clementine, whereas levels of GA(4) did not differ greatly. Levels of GA(9) were very low in both species. At petal fall, fruitlets of Satsuma and Clementine contained 65 and 13 picograms of GA(1), respectively. At this time, the application of 25 micrograms of paclobutrazol to fruits increased fruit abscission in both varieties. This effect was reversed by the simultaneous applications of 1 microgram of GA(3). GA(3) alone improved the set in Clementine (13x), but had little influence on Satsuma. Thus, seedless fruits of the self-incompatible Clementine mandarin may not have adequate GA levels for fruit set. Collectively, these results suggest that endogenous GA content in developing ovaries is the limiting factor controlling the parthenocarpic development of the fruits.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                agro
                Agrociencia Uruguay
                Agrociencia Uruguay
                Facultad de Agronomía - Instituto Nacional de Investigación Agropecuaria (Montevideo )
                2301-1548
                January 2010
                : 14
                : 1
                : 8-14
                Affiliations
                [1 ] Instituto Nacional de Investigación Agropecuaria (INIA) Uruguay
                [2 ] Universidad Politécnica de Valencia (UPV) España
                Article
                S2301-15482010000100002
                47fd2790-ea39-49c1-9afb-19fb1bd656e7

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Uruguay

                Self URI (journal page): http://www.scielo.edu.uy/scielo.php?script=sci_serial&pid=2301-1548&lng=en
                Categories
                AGRICULTURAL ECONOMICS & POLICY
                AGRICULTURAL ENGINEERING
                AGRICULTURE, DAIRY & ANIMAL SCIENCE
                AGRICULTURE, MULTIDISCIPLINARY
                AGRONOMY

                Animal agriculture,Agricultural engineering,General agriculture,Agricultural economics & Resource management,Horticulture
                citrus,cuajado,gibberellins,abscisic acid,carbohydrates,fruit set.,giberelinas,ácido abscísico,carbohidratos

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