SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection
      of Viable 
      <i>Brucella</i> Bacterium in Complex Matrix

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Abstract

Brucella is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify Brucella abortus in milk by our portable SERS device. Upon bacterial capture from samples (10 ⁴ cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection.

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Most cited references 44

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Targeting mechanisms of tailed bacteriophages

Bas Dutilh, Stan Brouns, Marnix Vlot … (2018)

Phages differ substantially in the bacterial hosts that they infect. Their host range is determined by the specific structures that they use to target bacterial cells. Tailed phages use a broad range of receptor-binding proteins, such as tail fibres, tail spikes and the central tail spike, to target their cognate bacterial cell surface receptors. Recent technical advances and new structure-function insights have begun to unravel the molecular mechanisms and temporal dynamics that govern these interactions. Here, we review the current understanding of the targeting machinery and mechanisms of tailed phages. These new insights and approaches pave the way for the application of phages in medicine and biotechnology and enable deeper understanding of their ecology and evolution.

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Economics of brucellosis impact and control in low-income countries.

Andrew McDermott, D. Grace, J. Zinsstag (2013)

Most data and evidence on the economic burden of brucellosis and the benefits of its control are from high-income and middle-income countries. However, the burden of brucellosis is greatest in low-income countries. This paper focuses on estimating the economic burdens of brucellosis in low-income countries in tropical Asia and Africa. The prospects for national, technically feasible, and economically viable, national brucellosis control programmes in most low-income countries are limited. However, some targeted control programmes will be beneficial and can probably be feasibly managed and provide good economic returns. More ambitious control will require a more general strengthening of Veterinary Services and livestock-sector capacity, using risk-management-based approaches.

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Deterministic aperiodic nanostructures for photonics and plasmonics applications

S.V. Boriskina, L. Dal Negro (2012)

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Author and article information

Contributors

Sharmila M. Mukhopadhyay: Role: Academic Editor

Journal

Journal ID (nlm-ta): Nanomaterials (Basel)

Journal ID (iso-abbrev): Nanomaterials (Basel)

Journal ID (publisher-id): nanomaterials

Title: Nanomaterials

Publisher: MDPI

ISSN (Electronic): 2079-4991

Publication date (Electronic): 31 March 2021

Publication date Collection: April 2021

Volume: 11

Issue: 4

Electronic Location Identifier: 886

Affiliations

[1 ]Institute of Applied Sciences and Intelligent Systems “E. Caianiello” of CNR, 80078 Pozzuoli, Italy; massimo.rippa@ 123456isasi.cnr.it (M.R.); riccardo.castagna@ 123456isasi.cnr.it (R.C.); domenico.sagnelli@ 123456isasi.cnr.it (D.S.); giovanna.fusco@ 123456izsmportici.it (G.F.)

[2 ]Istituto Zooprofilattico Sperimentale del Mezzogiorno (IZSM), 80055 Portici, Italy; giorgia.borriello@ 123456cert.izsmportici.it

[3 ]Institute of Photonics, Faculty of Science, Ningbo University, Ningbo 315211, China; zhoujun@ 123456nbu.edu.cn

Author notes

[* ]Correspondence: ambra.vestri@ 123456isasi.cnr.it (A.V.); lucia.petti@ 123456isasi.cnr.it (L.P.)

[†]

Authors contributed equally.

Author information

Ambra Vestri https://orcid.org/0000-0001-9877-7341

Giorgia Borriello https://orcid.org/0000-0002-4672-6793

Article

Publisher ID: nanomaterials-11-00886

DOI: 10.3390/nano11040886

PMC ID: 8067257

PubMed ID: 33807185

SO-VID: 4628a06c-52cf-408b-81cb-f2436fce86d9

License:

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( https://creativecommons.org/licenses/by/4.0/).

SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix

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Abstract

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Photoluminescent Nanomaterials

Most cited references 44

Targeting mechanisms of tailed bacteriophages

Economics of brucellosis impact and control in low-income countries.

Deterministic aperiodic nanostructures for photonics and plasmonics applications

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