Case Presentation
Dr. Reza Taslimi (Internist)
:A 34-year-old HIV positive-man was admitted to the hospital due to fever and abdominal
pain. Constant and generalized abdominal pain started 3 months prior to admission
(PTA) in upper quadrants of abdomen followed by mild odynophagia, early satiety and
more than 20 kg weight loss since then. Intermittent fever begun 7 days PTA and cough,
sputum and dyspnea started since 5 days PTA. His general condition deteriorated and
became drowsy 2-3 days PTA, so was referred to our center by patient’s accompanies.
Patient was a known case of intravenous heroin and a recent case of crack user. He
smoked cigarette more than 2 packs a day and drinks alcohol occasionally. On admission
day he looked toxic and lethargic. BP:95/60 mmHg, PR:128/min, RR:28/min, Temperature:41.5°c
and O2 saturation in room air :92%. Physical Exam (P/E) of skin revealed multiple
tattoos and some scars. Head and neck P/E were normal. P/E of heart showed tachycardia
without murmur. There were fine crackles in lower zone of left lung associated with
generalized wheezing. On abdominal examination, there was abdominal distension with
shifting dullness, hepatomegaly (8cm below costal margin) and generalized tenderness
without guarding or rebound. There was two plus pitting edema in lower extremities.
Peripheral lymphadenopathy was not detected. Patient was admitted to intensive care
unit. Abdominal Ultrasound showed liver enlargement with two large heterogeneous mass.
Common bile duct and portal vein diameters were normal. Gall bladder was normal and
moderate ascites was detected. In transthoracic echocardiography (TTE) ejection fraction
was normal and there was no vegetation. In upper GI endoscopy esophageal candidiasis
was seen. Colonoscopy was unremarkable, urine analysis was normal, and urine culture
and blood culture results were negative. Laboratory findings (Table 1) and ascitic
fluid analysis (Table 2) are presented. Chest X-ray (Figure 1 ) and abdominal CT scan
(Figures 2 and 3) were performed.
Table 1
Laboratory findings
WBC
14700/µl
BUN
7 mg/dl
LDH
1800 IU/L
PMN
82%
Cr
0.6 mg/dl
Serum protein
5.8 gr/dl
Lym
18%
Na
140 meq/l
Albumin
2.4 gr/dl
Hb
12 gr/dl
K
3.6 meq/l
AST
35 IU/L
Plt
257000/µl
Ca
8.2 mg/dl
ALT
23 IU/L
FBS
87 mg/dl
Ph
2.4 mg/dl
ALP
1018 IU/L
Direct Bilirubin
0.3 mg/dl
Total Bilirubin
0.8 mg/dl
GGT
80 IU/L
PTT
32 Sec
PT
12.5 Sec
ESR
29 mm/hr
αFP
1.5 ng/ml
CEA
1.2 ng/ml
CA 19-9
14 U/ml
CA 125
13 u/ml
HBs Ag
negative
HBc Ab
Negative
HCV Ab
negative
HIV Ab
positive
Table 2
Ascitic fluid analysis
color
yellow
Appearance
Semi turbid
WBC
170/mlPMN=58% , Lymph=42%
RBC
300/ml
Alb
1.5 gr/Serum Alb=2.4 gr/dl (SAAG=0.9)
Prot
3 gr/dl
LDH
850 IU/L
Glucose
67 mg/dl
Fig1
Ill defined consolidation is noted in left lower lobe.
Fig 2
Two hypodence masses in both liver lobes.
Fig 3
Large hypodence masses in liver causing hepatomegaly & pressure effect on portal veins
and stomach
Dr. Minoo Mohraz: May we review the imaging studies results first?
Radiological discussion
Hadi
Rokni(
Radiologist): In PA Chest X ray heart, mediastinum and bony thorax are normal. No
pleural effusion is noted. Ill defined consolidation is noted in left lower lobe without
obscuration of left heart border but with subtle obscuration of left hemidiaphragm
border.
Contrast enhanced abdominal CT scan in portal phase shows large hypodense masses in
both liver lobes causing hepatomegaly and pressure effect on portal veins, hepatic
veins and stomach.
Although multiple liver masses have a long list of differential diagnoses, but it
is more limited in AIDS patients. Mycobacterium avium intracellular (MAI) infections,
fungal infections like actinomycosis Israeli or blastomycosis can produce liver masses
and also lung infiltrates but in this case the masses are too large; although the
size cannot exclude these differential diagnoses. Lymphoma can produce large liver
masses and also lung infiltrates.
1
Kaposi sarcoma can produce liver masses but usually smaller masses with peripheral
periportal distribution, without or with mild enhancement and usually with skin or
mucosal involvements. Leiomyomatosis or peliosis hepatis which can be seen in AIDS
patients as a cause of liver masses also have smaller size and would be enhanced.
So primary hepatic lymphoma and MAI or fungal infections are the most probable diagnoses,
especially lymphoma.
2,3
RIZZI et al. presented the ultrasounds and CT scan findings of 26 patients with diagnosis
of AIDS and liver lymphoma. 10 patients had primary and 16 had secondary neoplasms
and they found that, majority of both primary and secondary liver lymphomas were multiple
lesions. Ultrasound findings showed hypoechoic lesions in 25 (96.1%) patients. In
the same study, CT scan showed that all lesions were hypodense with enhancing-ring
contrast in only six (23%) patients.
4
Our case presented with two large lobulated lesions which both have heterogenous echogenecity
in the ultrasound. In the same study, CT scan showed that all lesions were hypodense
with ring enhancing contrast in only six (23%) patients, the lesions in our case were
also hypodense and without significant enhancement.
Peixoto et al. reported 3 cases of primary non hodgkin’s lymphoma of liver. They found
out that all hepatic lesions showed target sign on ultrasound. In computed tomography
all cases showed a heterogeneous, hypodense mass with
a ring enhancement.
5
Clinical discussion
Dr. Minoo Mohraz (infectious disease specialist ): There is insufficient data on HIV
in east Mediterranean region. Estimated 460000 people living with HIV infection in
this region.
The number of new HIV infection in rejoin increased to 75000 in 2009.
6
In 2 countries (Djibouti and southern Sudan), HIV prevalence among pregnant women
exceeds 1%. HIV prevalence in other countries across the region remains low.
7
There is evidence of HIV prevalence over 5% among men having sex with men, and prevalence
2-4% among sex workers in some countries.
There are many causes for ascites. The most common cause is cirrhosis, which accounts
for approximately 80 percent of cases. Other causes include malignancy such as peritoneal
carcinomatosis, lymphoma with lymphatic obstruction; heart failure; infections such
as tuberculosis, pelvic inflammatory disease; end stage renal disease (ESRD); pancreatic
disease; pseudomyxoma peritonei; Meige’s syndrome and trauma.
8
Classification of ascites by serum-to-ascites albumin gradient (SAAG): Differential
diagnosis of high SAAG (≥1.1g/dl) consist of cirrhosis, congestive heart failure,
fulminant hepatic failure, fatty liver of pregnancy, liver metastases, portal vein
thrombosis, alcoholic hepatitis, Budd-Chiari syndrome, veno-occlusive disease, myxedema
and dialysis related ascites.
8
Differential diagnosis of low SAAG (<1.1g/dl) consist of nephrotic syndrome, peritoneal
carcinomatosis, biliary disease without cirrhosis, connective tissue disease (serosities),
tuberculosis and pelvic inflammatory disease (chlamydial or gonococcal).
Causes of hepatic disease in HIV infection:
These include: first: hepatitis including hepatitis B and C.
9
Other viral hepatitis is HDV, cytomegalovirus, Epstien-Barr virus, herpes simplex
virus, adenovirus, varicella-zoster virus, HIV. Also hepatotoxic drugs and ethanol
may damage liver.
Second: granulomatous inflammation including mycobacterial infection (Mycobacterium
avium complex, Mycobacterium tuberculosis, Other atypical mycobacteria ), fungal infection
(Histoplasma capsulatum, Cryptococcus neoformans, Coccidioides immitis, Candida albicans
), protozoa (Pneumocystis carinii, Toxoplasma gondii, Microsporida, Schistosoma, Cryptosporidium
parvum ).
10
Third: mass lesions including Kaposi’s sarcoma, non-Hodgkin’s lymphoma (NHL) and other
neoplasms.
11
Fourth: vascular lesions including peliosis hepatis.
12
Liver mass lesions in HIV positive persons:
The first mass lesion which is going to be explained is Kaposi sarcoma (K.S). One
third of all patients have cutaneous involvement. Abdominal pain, hepatosplenomegaly
and increased alkaline phosphates (ALP) are present.
13
Central necrosis is common in these lesions. The second one is NHL B cell type. 14%
of patients have liver involvement. There are 15 reports of primary liver NHL.
14
Fever, night sweat, weight loss and increased ALP are present. Multiple liver mass
larger than K.S may present.
15
Other visceral lymph nodes are involved in 90%. The other liver mass lesions which
can be seen in these patients are fibrosarcoma, chronic lymphocytic leukemia, leiomyoma,
hepatoma, cholangiocarcinoma, metastatic lesion and mixed solid-cystic masses such
as MAC, CMV, Fungi, Protozoa, gram-negative microorganisms and staphylococci aureus
.
HIV associated lymphoproliferative disorders:
HIV associated lymphoma affects 5-10% of HIV infected individuals. The vast majority
of them are systemic B-cell type NHL. 20% involves CNS and consists primary CNS lymphoma.
Primary effusion lymphoma accounting for less than 1 to 4 percent of cases.
16
HIV associated Burkitt lymphoma is one of the common forms of lymphoma in this disease
and 25-40% are EBV associated.
Dr. Mohraz’s diagnosis:
lymphoma.
Pathology discussion
Dr. Sotoudeh (pathologist):
Histological features and immumohistochemical (IHC) studies performed on the liver
needle biopsy specimen were diagnostic for Burkitt lymphoma in this patient.
Association of HIV infection and AIDS with lymphoproliferative disorders and some
other types of malignancies has been recognized since 1981, as early as HIV associated
diseases were defined. Natural history of HIV and AIDS related malignancies has changed
since the introduction of more effective anti-viral therapy for the patients.
17
Patients with HIV infection are in definite risk of development of lymphoma. 5-10%
of these patients are believed to be diagnosed with lymphoid malignancies.The risk
increases constantly in the survivors and may reach up to 29% by the third year of
antiviral therapy. Multiple pathways are possibly involved in the pathogenesis of
lymphoma in HIV patients. Most of the lymphomas detected in these patients are high
grade diffuse large B-cell type similar to the tumors seen in organ transplant recipients.
However, unlike the tumors in transplanted patients, EBV DNA sequence is much less
frequently found. EBV associated large B-cell lymphoma is mostly seen in HIV patients
with primary CNS lymphomas.
18
Burkitt lymphoma (diffuse small non-cleaved lymphoma) is the second most common lymphoma
in HIV patients and is a highly aggressive tumor. 30-50% of these tumors are reported
to be associated with EBV infection.
19
The patient presented in this clinic pathological conference is a typical case of
HIV associated primary hepatic Burkitt lymphoma.
The tumor has been multifocal in liver but extensive imaging and other works up could
not detect any lymph adenopathy or other sites of involvement by the tumor.The liver
biopsy showed a tumoral tissue composed of small cells with high nucleo-cytoplasmic
ratio and small amount of cytoplasm (Figure 4).
Fig 4
Tumor cells are small but have relatively large nuclei
and small amount of cytoplasm. Macrophages shown
by arrow heads are frequently seen among the tumor
cells. The reason for the so-called starry sky in low
power microscopy.
The tumor cells showed brisk mitotic activity forming sheets that occupied the liver
paren-chyma. Low power examination showed the so- called starry sky appearance. IHC
staining of the tumor cells showed reaction for LCA, CD20 and CD10. Almost all (98%)
of tumor cell nuclei showed reaction for Ki67. No reaction could be demonstrated in
tumor cells by antibodies against cytokeratin, CD3, CD99, CD23, Bcl2 and CD34 (Figure
5). PCR for CMV was inconclusive.
Fig 5
IHC studies show strong and diffuse reaction of the tumor cells for LCA and CD20.
Moderate positive reaction of tumor cells for CD10 is observed. Reaction of 98% of
tumor cell nuclei for Ki67 protein indicates a high proliferation activity in the
tumor cells and corresponds to the large number of mitotic figures observed in the
histological sections.
Final Pathological Diagnosis
Primary hepatic Burkitt lymphoma in a HIV patient
CONFLFLICT OF INTEREST
The authors declare no conflict of interest related to this work.