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      MYB70 modulates seed germination and root system development in Arabidopsis

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          Summary

          Crosstalk among ABA, auxin, and ROS plays critical roles in modulating seed germination, root growth, and suberization. However, the underlying molecular mechanisms remain largely elusive. Here, MYB70, a R2R3-MYB transcription factor was shown to be a key component of these processes in Arabidopsis thaliana. myb70 seeds displayed decreased sensitivity, while MYB70-overexpressing OX70 seeds showed increased sensitivity in germination in response to exogenous ABA through MYB70 physical interaction with ABI5 protein, leading to enhanced stabilization of ABI5. Furthermore, MYB70 modulates root system development (RSA) which is associated with increased conjugated IAA content and H 2O 2/O 2 ⋅− ratio but reduced root suberin deposition, consequently affecting nutrient uptake. In support of these data, MYB70 positively regulates the expression of auxin conjugation-related GH3, while negatively peroxidase-encoding and suberin biosynthesis-related genes. Our findings collectively revealed a previously uncharacterized component that modulates ABA and auxin signaling pathways, H 2O 2/O 2 ⋅− balance, and suberization, consequently regulating RSA and seed germination.

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          Highlights

          • •

            MYB70 regulates seed germination by enhancing ABA signaling via interaction with ABI5

          • •

            MYB70 activates the IAA conjugation process by upregulating GH3 genes expression

          • •

            MYB70 mediates root growth via repression of PER genes

          • •

            MYB70 negatively regulates suberin biosynthesis in roots

          Abstract

          Biological sciences; Cell biology; Plant biology; Plant histology; Plant Genetics

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          Most cited references77

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          Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2

          Michael Love, Wolfgang Huber, Simon Anders (2014)
          In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence of outliers require a suitable statistical approach. We present DESeq2, a method for differential analysis of count data, using shrinkage estimation for dispersions and fold changes to improve stability and interpretability of estimates. This enables a more quantitative analysis focused on the strength rather than the mere presence of differential expression. The DESeq2 package is available at http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html. Electronic supplementary material The online version of this article (doi:10.1186/s13059-014-0550-8) contains supplementary material, which is available to authorized users.
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            Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants

            Roger Hellens, Andrew Allan, Ellen N Friel … (2006)
            Background We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited to transient gene expression, by removing elements of conventional binary vectors necessary for stable transformation such as transformation selection genes. Results We give an example of expression of heme-thiolate P450 to demonstrate effectiveness of this system. We have also designed vectors that take advantage of a dual luciferase assay system to analyse promoter sequences or post-transcriptional regulation of gene expression. We have demonstrated their utility by co-expression of putative transcription factors and the promoter sequence of potential target genes and show how orthologous promoter sequences respond to these genes. Finally, we have constructed a vector that has allowed us to investigate design features of hairpin constructs related to their ability to initiate RNA silencing, and have used these tools to study cis-regulatory effect of intron-containing gene constructs. Conclusion In developing a series of vectors ideally suited to transient expression analysis we have provided a resource that further advances the application of this technology. These minimal vectors are ideally suited to conventional cloning methods and we have used them to demonstrate their flexibility to investigate enzyme activity, transcription regulation and post-transcriptional regulatory processes in transient assays.
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              Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid.

              Paul E Staswick, Bogdan Serban, Martha Rowe … (2005)
              Substantial evidence indicates that amino acid conjugates of indole-3-acetic acid (IAA) function in auxin homeostasis, yet the plant enzymes involved in their biosynthesis have not been identified. We tested whether several Arabidopsis thaliana enzymes that are related to the auxin-induced soybean (Glycine max) GH3 gene product synthesize IAA-amino acid conjugates. In vitro reactions with six recombinant GH3 enzymes produced IAA conjugates with several amino acids, based on thin layer chromatography. The identity of the Ala, Asp, Phe, and Trp conjugates was verified by gas chromatography-mass spectrometry. Insertional mutations in GH3.1, GH3.2, GH3.5, and GH3.17 resulted in modestly increased sensitivity to IAA in seedling root. Overexpression of GH3.6 in the activation-tagged mutant dfl1-D did not significantly alter IAA level but resulted in 3.2- and 4.5-fold more IAA-Asp than in wild-type seedlings and mature leaves, respectively. In addition to IAA, dfl1-D was less sensitive to indole-3-butyric acid and naphthaleneacetic acid, consistent with the fact that GH3.6 was active on each of these auxins. By contrast, GH3.6 and the other five enzymes tested were inactive on halogenated auxins, and dfl1-D was not resistant to these. This evidence establishes that several GH3 genes encode IAA-amido synthetases, which help to maintain auxin homeostasis by conjugating excess IAA to amino acids.
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                Author and article information

                Contributors
                Lam-Son Tran
                Jin Xu
                Journal
                Journal ID (nlm-ta): iScience
                Journal ID (iso-abbrev): iScience
                Title: iScience
                Publisher: Elsevier
                ISSN (Electronic): 2589-0042
                Publication date PMC-release: 07 October 2021
                Publication date Collection: 19 November 2021
                Publication date (Electronic): 07 October 2021
                Volume: 24
                Issue: 11
                Electronic Location Identifier: 103228
                Affiliations
                [1 ]CAS Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China
                [2 ]College of Horticulture, Shanxi Agricultural University, Taigu 030801, China
                [3 ]Institute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam
                [4 ]Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA
                [5 ]State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062, China
                [6 ]Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Menglun, Mengla 666303, China
                Author notes
                [∗ ]Corresponding author son.tran@ 123456ttu.edu
                [7]

                Lead contact

                Article
                Publisher Item ID: S2589-0042(21)01196-2 Publisher ID: 103228
                DOI: 10.1016/j.isci.2021.103228
                PMC ID: 8551079
                PubMed ID: 34746697
                SO-VID: 0f3613bf-316d-408e-920c-4cf6325dd442
                Copyright © © 2021 The Author(s)
                License:

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                Date received : 11 June 2021
                Date revision received : 25 August 2021
                Date accepted : 1 October 2021
                Categories
                Subject: Article

                Keywords: biological sciences,cell biology,plant biology,plant histology,plant genetics
                Data availability:
                Keywords: biological sciences, cell biology, plant biology, plant histology, plant genetics

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