Studies on Antioxidant Capacity in Selenium-Deprived the Choko Yak in the Shouqu Prairie

Inter-annual dynamics of grassland yield of the Three Rivers Headwaters Region of Qinghai-Tibet Plateau of China in 1988-2005 was analyzed using the GLO-PEM model, and the herbage supply function was evaluated. The results indicate that while grassland yield in the region showed marked inter-annual fluctuation there was a trend of increased yield over the 18 years of the study. This increase was especially marked for Alpine Desert and Alpine Steppe and in the west of the region. The inter-annual coefficient of variation of productivity increased from the east to the west of the region and from Marsh, Alpine Meadow, Alpine Steppe, Temperate Steppe to Alpine Desert grasslands. Climate change, particularly increased temperatures in the region during the study period, is suggested to be the main cause of increased grassland yield. However, reduced grazing pressure and changes to the seasonal pattern of grazing could also have influenced the grassland yield trend. These findings indicate the importance of understanding the function of the grassland ecosystems in the region and the effect of climate change on them especially in regard to their use to supply forage for animal production. Reduction of grazing pressure, especially during winter, is indicated to be critical for the restoration and sustainable use of grassland ecosystems in the region.

Selenium (Se) is known to have antagonistic effects against lead (Pb) toxicity in animal. The aim of this study was to evaluate the roles of mitochondrial dynamics on Pb-induced apoptosis in the chicken kidney and investigate the antagonistic effect of Se. In the present study, brown layer chickens were randomly allocated to four groups, and each group were exposed to a basic diet (0.2 mg kg(-1) Se and 0.5 mg kg(-1) Pb), a Se-adequate diet (1 mg kg(-1) Se and 0.5 mg kg(-1) Pb), a Se and Pb compound diet (1 mg kg(-1) Se and 350 mg kg(-1) Pb) or a Pb supplemented diet (0.2 mg kg(-1) Se and 350 mg kg(-1) Pb). On the 90(th) day, the kidney was removed to determine the activities of mitochondrial respiratory chain complexes, ATPases and oxidative indexes. The expression levels of mitochondrial dynamics and apoptosis-related genes were also determined. The results showed that Pb treatment significantly decreased the activities of mitochondrial complexes and ATPases, and increased oxidative stress, and mitochondrial dynamics and anti-apoptosis-related genes had a lower expression, whereas mitochondrial pro-apoptosis related genes presented higher expressions in the Pb group compared with control group (P < 0.05). However, the co-treatment of Se and Pb significantly alleviated those changes compared with the Pb group (P < 0.05). In conclusion, we speculated that Pb could increase the oxidative stress and promote the apoptosis via regulating mitochondrial dynamics and apoptosis-related genes, and Se exhibited antagonistic roles against the Pb-induced apoptosis in the kidney of chickens.

Selenium is an essential dietary micronutrient. Ingested selenium is absorbed by the intestines and transported to the liver where it is mostly metabolized to selenocysteine (Sec). Sec is then incorporated into selenoproteins, including selenoprotein P (SELENOP), which is secreted into plasma and serves as a source of selenium to other tissues of the body. Herein, we provide an overview of the biology of selenium from its absorption and distribution to selenoprotein uptake and degradation, with a particular focus on the latter. Molecular mechanisms of selenoprotein degradation include the lysosome-mediated pathway for SELENOP and endoplasmic reticulum-mediated degradation of selenoproteins via ubiquitin-activated proteasomal pathways. Ubiquitin-activated pathways targeting full-length selenoproteins include the peroxisome proliferator-activated receptor gamma-dependent pathway and substrate-dependent ubiquitination. An alternate mechanism is utilized for truncated selenoproteins, in which cullin-RING E3 ubiquitin ligase 2 targets the defective proteins for ubiquitin-proteasomal degradation. Selenoproteins, particularly SELENOP, may have their Sec residues reutilized for new selenoprotein synthesis via Sec decomposition. This review will explore these aspects in selenium biology, providing insights to knowledge gaps that remain to be uncovered.