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Complex Carbohydrates
[39] Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells
edited_book
Author(s):
Yu-Li Wang
,
Robert J. Pelham
Publication date
(Print):
1998
Publisher:
Elsevier
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Gamma Delta T cells
Author and book information
Book Chapter
Publication date (Print):
1998
Pages
: 489-496
DOI:
10.1016/S0076-6879(98)98041-7
SO-VID:
a9e91f2b-f4ea-4eec-b013-f30b27ac6ccd
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Book chapters
pp. ii
Series Page
pp. iv
Copyright
pp. xi
Contributors
pp. xix
Preface
pp. xv
Preface
pp. 1
Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome
pp. 1
Atlas of the Radical SAM Superfamily: Divergent Evolution of Function Using a “Plug and Play” Domain
pp. 1
Chemical Posttranslational Modification with Designed Rhodium(II) Catalysts
pp. 3
[1] Selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids
pp. 3
[1] Production of single-stranded plasmid DNA
pp. 3
Preparation of nucleosome core particle from recombinant histones
pp. 3
[1] DNA bending, flexibility, and helical repeat by cyclization kinetics
pp. 3
[1] Preparation of monoclonal antibodies: Strategies and procedures
pp. 3
[1] Isolation and purification of cyanobacteria
pp. 3
[1] Reflections
pp. 3
Epitope tagging: General method for tracking recombinant proteins
pp. 3
Use of Chemical Shifts in Macromolecular Structure Determination
pp. 3
[1] Concept and early development of solid-phase peptide synthesis
pp. 3
[1] Measurement of molecular weights by electrophoresis on SDS-acrylamide gel
pp. 3
[1] Optical measurements of calcium signals in mammalian presynaptic terminals
pp. 3
[1] Structure and deformation properties of red blood cells: Concepts and quantitative methods
pp. 3
[1] Introduction to the plasma lipoproteins
pp. 3
Enzyme Discovery: Enzyme Selection and Pathway Design
pp. 3
[1] Analysis of sugars found in glycoproteins
pp. 4
[2]Scanning microcalorimetry in studying temperature-induced changes in proteins
pp. 5
[1] Extraction of tissue lipids with a solvent of low toxicity
pp. 5
[2] Introduction to avidin-biotin technology
pp. 8
[2] Thiol/disulfide exchange equilibria and disulfidebond stability
pp. 10
[2] Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis
pp. 14
[3] Applications of avidin-biotin technology: Literature survey
pp. 19
Synthetic probe development for measuring single or few-cell activity and efflux
pp. 19
[2] High-efficiency full-length cDNA cloning
pp. 19
Preparation of crystalline, amorphous, and dyed cellulase substrates
pp. 20
[2] Titration of trypsin, plasmin, and thrombin with p-nitrophenyl p′-guanidinobenzoate HCl
pp. 22
[3] Purification of Escherichia coli DNA polymerase III holoenzyme
pp. 25
Sulfane sulfur
pp. 26
[2] Characterization of carbohydrate units of glycoproteins
pp. 33
[2] Preparation and characterization of the different types of collagen
pp. 34
[4] Circular dichroism
pp. 36
[4] Isolation of sarcoplasmic reticulum fractions referable to longitudinal tubules and functional terminal cisternae from rabbit skeletal muscle
pp. 38
[4] Approaches to investigating nuclear genes that function in chloroplast biogenesis in land plants
pp. 38
[2] Protein kinase catalytic domain sequence database: Identification of conserved features of primary structure and classification of family members
pp. 38
[5] Diversity of glutathione peroxidases
pp. 41
[7] Mitochondrial respiratory control and the polarographic measurement of ADP : O ratios
pp. 41
[3] PIR-International protein sequence database
pp. 43
[3] Macromolecules and water: Probing with osmotic stress
pp. 43
Enzymatic activity in single cells
pp. 43
Microbiological assay for serum, plasma, and red cell folate using cryopreserved, microtiter plate method
pp. 44
Spectrophotometric assay of thiols
pp. 44
[3] Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance
pp. 44
[4] Standard Fmoc protocols
pp. 48
[8] Preparation of retinal rod outer segments
pp. 49
[8] Reassessment of Ellman's reagent
pp. 50
[6] Quantitation of protein
pp. 50
[4] Preparation and use of nuclease-treated rabbit reticulocyte lysates for the translation of eukaryotic messenger RNA
pp. 51
[5] Avidin and streptavidin
pp. 51
[5] Synthesis of small RNAs using T7 RNA polymerase
pp. 51
[6] Yeast expression of animal and plant P450s in optimized redox environments
pp. 52
[3] Cobalt as probe and label of proteins
pp. 53
[3] Hydrogen ion buffers
pp. 55
[7] Preparation of myosin and its subfragments from rabbit skeletal muscle
pp. 57
[5] Deoxyribose assay for detecting hydroxyl radicals
pp. 59
[6] Determination of cystine as cysteic acid
pp. 59
Single-cell assays using integrated continuous-flow microfluidics
pp. 61
Key Factors for Successful Generation of Protein–Fragment Structures
pp. 62
[3] Protein kinase phosphorylation site sequences and consensus specificity motifs: Tabulations
pp. 63
[4] Plasmid transformation of Escherichia coli and other bacteria
pp. 63
Production and spectroscopic characterization of lytic polysaccharide monooxygenases
pp. 63
[5] Rapid and sensitive sequence comparison with FASTP and FASTA
pp. 67
FASTBAC-Seq: Functional Analysis of Toxin–Antitoxin Systems in Bacteria by Deep Sequencing
pp. 68
[3] Culturing methods for cyanobacteria
pp. 70
Semisynthesis of Proteins Containing Selenocysteine
pp. 71
[4] Use of denaturing gradient gel electrophoresis to study conformational transitions in nucleic acids
pp. 73
Screening Library Design
pp. 73
[12] Colorimetric analysis of sugars
pp. 79
[2] Measurement of homo- and heteronuclear J couplings from quantitative J correlation
pp. 79
[4] Genetic and microbiological research techniques for Neurospora crassa
pp. 83
[6] Properties of solid supports
pp. 85
Determination of low-molecular-weight thiols using monobromobimane fluorescent labeling and high-performance liquid chromatography
pp. 85
[6] New colorimetric methods of sugar analysis
pp. 85
[4] The preparation of antigenic Hapten-Carrier conjugates: A survey
pp. 87
Methods for measuring cellulase activities
pp. 87
Lignin determination
pp. 90
Resolution of phase ambiguity in macromolecular crystallography
pp. 91
[6] Genetic approaches to study of biofilms
pp. 94
[15] Isolation of liver or kidney mitochondria
pp. 95
Single-cell activity screening in microfluidic droplets
pp. 95
[12] Determination of total protein
pp. 97
In Vivo Platforms for Terpenoid Overproduction and the Generation of Chemical Diversity
pp. 99
[13] Bacterial Membranes
pp. 101
High-performance liquid chromatography of thiols and disulfides: Dinitrophenol derivatives
pp. 101
Enzymatic methods for quantification of lipoprotein lipids
pp. 102
[7] 1,1′-Carbonyldiimidazole-mediated immobilization of enzymes and affinity ligands
pp. 103
[6] Statistical analysis of enzyme kinetic data
pp. 106
Preparation of stable isotopically labeled folates for in vivo investigation of folate absorption and metabolism
pp. 108
[8] Solid state nuclear magnetic resonance of lipid bilayers
pp. 108
[12] Genetic and chemical tools for investigating signaling processes in biofilms
pp. 108
[8] Mass spectrometry of carbohydrate-containing biopolymers
pp. 110
[11] Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates
pp. 113
The microfluidic capture of single breast cancer cells for multi-drug resistance assays
pp. 113
[5] General carotenoid methods
pp. 114
[8] SRS: Information retrieval system for molecular biology data banks
pp. 115
[10] Assay of inorganic phosphate, total phosphate and phosphatases
pp. 115
Electron Spin Resonance (EPR) in Drosophila and General Anesthesia
pp. 116
[11] Coupled assay of Na+,K+-ATPase activity
pp. 117
Chapter 6 Using Expressed Protein Ligation to Probe the Substrate Specificity of Lantibiotic Synthetases
pp. 123
[6] Correction for liquid junction potentials in patch clamp experiments
pp. 125
[6] Efficient site-directed mutagenesis using uracil-containing DNA
pp. 126
[8] Handles for solid-phase peptide synthesis
pp. 127
[7] Molecular crowding: Analysis of effects of high concentrations of inert cosolutes on biochemical equilibria and rates in terms of volume exclusion
pp. 129
Single-cell proteolytic activity measurement using microfluidics for rare cell populations
pp. 129
[8] Singular value decomposition: Application to analysis of experimental data
pp. 131
[9] Applications of network BLAST server
pp. 132
[9] Quantitative DNase footprint titration: A method for studying protein-DNA interactions
pp. 134
[7] Optical-trap force transducer that operates by direct measurement of light momentum
pp. 138
Phosphorylation and identification of phosphorylated forms of basic fibroblast growth factor
pp. 139
[10] Gangliosides: Structure, isolation, and analysis
pp. 139
[9] Infrared methods for study of hemoglobin reactions and structures
pp. 139
[19] 4-Hydroxyphenylpyruvate dioxygenase from human liver
pp. 139
Identification and Characterization of Modification Enzymes by Biochemical Analysis of the Proteome
pp. 140
[8] Catabolism and turnover of collagens: Collagenases
pp. 141
[10] Entrez: Molecular biology database and retrieval system
pp. 142
Measurement of nitrate and nitrite in biological samples using nitrate reductase and Griess reaction
pp. 143
Escherichia coli Ribonuclease III: Affinity Purification of Hexahistidine-Tagged Enzyme and Assays for Substrate Binding and Cleavage
pp. 145
Microfluidic technology for investigation of protein function in single adherent cells
pp. 146
[12] Expression of G-protein a subunits in Escherichia coli
pp. 147
[12] Preparation of extracts from prokaryotes
pp. 149
Cysteamine and cystamine
pp. 151
Dynamic Assessment of Nitration Reactions In Vivo
pp. 151
Synergy of Molecular Dynamics and Isothermal Titration Calorimetry in Studies of Allostery
pp. 151
[7] The use of carbodiimides in the preparation of immunizing conjugates
pp. 152
[9] Gel electrophoresis of restriction fragments
pp. 153
[9] Culture of preimplantation embryos
pp. 154
[25] d-Fructose dehydrogenase from Gluconobacter industrius, membrane-bound
pp. 155
[6] Sequential flotation ultracentrifugation
pp. 157
Interactive computer graphics: FRODO
pp. 162
GeneChips in Stem Cell Research
pp. 162
[11] Applying motif and profile searches
pp. 164
[22] Preparation of plasma-membrane fragments from mouse ascites tumor cells
pp. 164
[18] Purification of muscle actin
pp. 173
Nanokits for the electrochemical quantification of enzyme activity in single living cells
pp. 175
Survival Surgery for Coronary Occlusion and Reoxygenation in a Rodent Model
pp. 175
Dual-Focus Fluorescence Correlation Spectroscopy
pp. 175
[16] Nitrotyrosine as biomarker for reactive nitrogen species
pp. 175
[23] Phosphoacetylglucosamine mutase from Neurospora
pp. 176
[10] Elution of DNA from agarose gels after electrophoresis
pp. 178
Magnetic Relaxation Dispersion Studiesof Biomolecular Solutions
pp. 179
[24] Phosphoacetylglucosamine mutase from pig submaxillary gland
pp. 179
[12] DNA arrays for analysis of gene expression
pp. 181
[8] Single vertical spin density gradient ultracentrifugation
pp. 182
[18] Ferrous ion oxidation in presence of ferric ion indicator xylenol orange for measurement of hydroperoxides
pp. 183
Generation of a Metagenomics Proximity Ligation 3C Library of a Mammalian Gut Microbiota
pp. 183
[13] Evolutionary families of metallopeptidases
pp. 185
[26] N-acylglucosamine 6-phosphate 2-epimerase
pp. 187
Preparation of Phosphorylated Proteins for NMR Spectroscopy
pp. 190
Metal chelation of polyphenols
pp. 191
Design of an automated capillary electrophoresis platform for single-cell analysis
pp. 192
[8] Hysteretic enzymes
pp. 194
[15] Preparation of extracts from higher eukaryotes
pp. 195
[109] Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA
pp. 197
[19] Performic acid oxidation
pp. 198
[10] Disulfide bond formation in peptides
pp. 199
[21] Thioredoxin and thioredoxin reductase
pp. 199
[20] Reduction and S-carboxymethylation of proteins
pp. 201
[19] Antibodies that recognize nitrotyrosine
pp. 201
Reannotation of Genomes by Means of Proteomics Data
pp. 201
[29] N-acylneuraminic (sialic) acid 9-phosphate synthetase
pp. 202
[20] Enzymatic and immunological measurements of soluble and membrane-bound phospholipid-hydroperoxide glutathione peroxidase
pp. 203
[16] Isolation of subcellular organelles
pp. 204
Nuclear Magnetic Resonance Methods for Quantifying Microsecond-to-Millisecond Motions in Biological Macromolecules
pp. 206
[25] Hydroxysteroid sulfotransferase
pp. 208
[31] CMP-sialic acid synthetase (cytidine-5′-monophospho-sialic acid synthetase)
pp. 208
[11] Calculation of protein conformation from circular dichroism
pp. 212
[16] Carbohydrate antigens: Coupling of carbohydrates to proteins by diazonium and phenylisothiocyanate reactions
pp. 213
[9] Delipidation of plasma lipoproteins
pp. 215
[12] Genetic engineering of the cyanobacterial chromosome
pp. 219
High Content, Phenotypic Assays and Screens for Compounds Modulating Cellular Processes in Primary Neurons
pp. 219
[20] Isolation of RNA using guanidinium salts
pp. 220
[23] Measurement of equilibrium midpoint potentials of thiol/disulfide regulatory groups on thioredoxinactivated chloroplast enzymes
pp. 220
[15] Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper
pp. 223
Microelectrophoretic single-cell measurements with microfluidic devices
pp. 227
[15] Effective protein sequence comparison
pp. 228
[15] Libraries of peptides and proteins displayed on filamentous phage
pp. 229
[23] Oxidative chemistry of peroxynitrite
pp. 237
Quantitation of nitrate and nitrite in extracellular fluids
pp. 238
[27] The cyanogen bromide reaction
pp. 238
Lignin peroxidase of Phanerochaete chrysosporium
pp. 241
[19] Receptor-mediated endocytosis of low-density lipoprotein in cultured cells
pp. 243
Chemical probes for spatially resolved measurement of active enzymes in single cells
pp. 243
[17] A rapid alkaline extraction method for the isolation of plasmid DNA
pp. 245
[16] Purification of proteins using polyhistidine affinity tags
pp. 245
[45] Cytochrome oxidase from beef heart mitochondria
pp. 245
[16] Plasmids of Escherichia coli as cloning vectors
pp. 248
[38] UDP-glucose pyrophosphorylase (crystalline) from liver
pp. 250
[15] High-resolution polyacrylamide gel electrophoresis of fluorophore-labeled reducing saccharides
pp. 250
[25] Nitric-oxide synthase assays
pp. 251
[24] Membrane lipids
pp. 252
Stereochemically restrained refinement of macromolecular structures
pp. 252
[16] Splice junctions, branch point sites, and exons: Sequence statistics, identification, and applications to genome project
pp. 253
[24] Use of specific inhibitors on the mitochondrial bc1 complex
pp. 255
[18] Hybridization of denatured RNA transferred or dotted to nitrocellulose paper
pp. 256
[17] Analysis of protein aggregation kinetics
pp. 259
[16] Discovering and understanding genes in human DNA sequence using GRAIL
pp. 263
Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry
pp. 264
An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts
pp. 264
[23] Production of phosphorylation state-specific antibodies
pp. 264
Manganese peroxidase of Phanerochaete chrysosporium: Purification
pp. 266
[15] Maintenance of Xenopus laevis and oocyte injection
pp. 266
[19] Isolation of multigene families and determination of homologies by filter hybridization methods
pp. 266
[14]Determination and analysis of urea and guanidine hydrochloride denaturation curves
pp. 267
[27] Translation in cell-free systems
pp. 274
[18] Quantification of β-sheet amyloid fibril structures with thioflavin T
pp. 278
[20] Use of rapid kinetics methods to study the assembly of the diferric-tyrosyl radical cofactor of E. coli ribonucleotide reductase
pp. 279
[241 Total antioxidant status in plasma and body fluids
pp. 280
[12] Mass spectrometry of peptides and proteins by matrix-assisted ultraviolet laser desorption/ionization
pp. 281
[17] Predicting optimal and suboptimal secondary structure for RNA
pp. 281
“NONOates” (1-substituted diazen-1-ium-1,2-diolates) as nitric oxide donors: Convenient nitric oxide dosage forms
pp. 281
Formation and isomerization of disulfide bonds in proteins: Protein disulfide-isomerase
pp. 283
[29] Glutaredoxin
pp. 285
Overall low molecular weight antioxidant activity of biological fluids and tissues by cyclic voltammetry
pp. 287
Eosinophil Cationic Protein
pp. 290
[13] Resolution-enhanced fourier transform infrared spectroscopy of enzymes
pp. 291
[31] Phycobiliproteins
pp. 292
[17] Binary ti vectors for plant transformation and promoter analysis
pp. 293
Electroosmotic extraction coupled to mass spectrometry analysis of metabolites in live cells
pp. 293
[21] β-Galactosidase gene fusions for analyzing gene expression in Escherichia coli and yeast
pp. 299
[19] In Vitro packaging of λ and cosmid DNA
pp. 300
[13] Fluorescence resonance energy transfer
pp. 301
[16] Nonlinear least-squares analysis
pp. 303
Transcriptomic Approaches for Studying Quorum Sensing in Vibrio cholerae
pp. 304
[27] Isolation of human cell lines lacking mitochondrial DNA
pp. 304
[22] absorbance melting curves of RNA
pp. 305
Disulfide bond formation in proteins
pp. 305
Analysis of von Hippel–Lindau Tumor Suppressor as a Mediator of Cellular Oxygen Sensing
pp. 309
[56] DT diaphorase
pp. 312
[29] Identification of carotenoid pigments in birds
pp. 315
[19] Assay of peroxisomal β-oxidation of fatty acids
pp. 316
[33] First-strand cDNA synthesis primed with oligo(dT)
pp. 317
[26] High-resolution video and digital-enhanced differential interference contrast light microscopy of cell division in budding yeast
pp. 318
[34] Complementary chromatic adaptation: Physiological conditions and action spectra
pp. 319
Binding of flavonoids to plasma proteins
pp. 324
[58] Formation of UDP-acetylmuramyl peptides
pp. 324
[28] Cleavage at aspartic acid
pp. 325
[44] Glutathione peroxidase
pp. 327
Defined Conditions for Neural Commitment and Differentiation
pp. 329
[25] Detection, isolation, and characterization of siderophores
pp. 330
[124] Use of cesium sulfate for equilibrium density gradient centrifugation
pp. 330
Redox control of enzyme activities by thiol/disulfide exchange
pp. 331
Methods for Controlled Protein Depletion to Study Protein Function during Meiosis
pp. 331
[34] Ras-Raf interaction: Two-hybrid analysis
pp. 333
[21] Phage display for selection of novel binding peptides
pp. 342
Cellulases in Phaseolus vulgaris
pp. 345
[32] Phospholipase activity of milk lipoprotein lipase
pp. 346
[37] Carbonyl assays for determination of oxidatively modified proteins
pp. 349
[20] Suppression subtractive hybridization: A versatile method for identifying differentially expressed genes
pp. 353
[18] Fluorescence resonance energy transfer and nucleic acids
pp. 353
[25] Mixed micelle assay of protein kinase C
pp. 354
[62] Animal sialic acid transferases (sialyl-transferases)
pp. 357
[38] Oxidative damage to proteins: Spectrophotometric method for carbonyl assay
pp. 357
[27] Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples
pp. 360
[27] Cell water viscosity
pp. 361
[22] Detection of messenger RNA by in Situ hybridization to tissue sections and whole mounts
pp. 363
[12] Chemical shifts as a tool for structure determination
pp. 363
[29] Analysis of repeating DNA sequences by reassociation
pp. 366
Mammalian sulfur amino acid metabolism: An overview
pp. 372
[17] Genetics of Caulobacter crescentus
pp. 373
[48] Assay of glutathione, glutathione disulfide, and glutathione mixed disulfides in biological samples
pp. 376
[49] Nucleoside diphosphokinase from human erythrocytes
pp. 377
Yeast suppressor mutations and transfer RNA processing
pp. 377
[29] Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis
pp. 377
Dityrosine: In vitro production and characterization
pp. 378
[31] Computer programs for calculating total from specified free or free from specified total ionic concentrations in aqueous solutions containing multiple metals and ligands
pp. 378
[17] Time-resolved fluorescence measurements
pp. 378
[32] Pseudomonas aeruginosa lectins
pp. 380
[41] Measurement of protein thiol groups and glutathione in plasma
pp. 382
[41] Preparation and assay of superioxide dismutases
pp. 383
[22] Using CLUSTAL for multiple sequence alignments
pp. 386
[31] Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons
pp. 387
[66] ADP-glucose: α-1,4-glucan glucosyltransferases (starch synthetases and related enzymes) from plants
pp. 387
[42] Detection and quantification of phosphotyrosine in proteins
pp. 390
[37] Protein phosphatase-1 and protein phosphatase-2A from rabbit skeletal muscle
pp. 391
[36] Immunoaffinity purification of soluble guanylyl cyclase
pp. 391
Analysis of Meiotic Double-Strand Break Initiation in Mammals
pp. 392
[36] Purification of a plasma membrane-bound adenosine triphosphatase from plant roots
pp. 395
RNase H1 of Saccharomyces cerevisiae: Methods and Nomenclature
pp. 395
[67] α-1,4-glucan: α-1,4-glucan 6-glycosyltransferase from mammalian muscle
pp. 397
[37] Copurification of atrial natriuretic peptide receptor and particulate guanylyl cyclase
pp. 398
[51] Assays for differentiation of glutathione S-Transferases
pp. 399
[36] High-sensitivity sequencing with a gas-phase sequenator
pp. 400
[29] Osmotic stress for the direct measurement of intermolecular forces
pp. 403
[38] Antioxidant effects of carotenoids in Vivo and in Vitro: An overview
pp. 405
Measurement of invasion by gentamicin resistance
pp. 405
[23] Buffers of constant ionic strength for studying pH-dependent processes
pp. 406
[29] Mitochondrial membrane potential monitored by JC-1 dye
pp. 411
[23] Redox potentiometry: Determination of midpoint potentials of oxidation-reduction components of biological electron-transfer systems
pp. 413
The Synthesis of 1,2-cis-Amino Containing Oligosaccharides Toward Biological Investigation
pp. 414
[45] Glutamine binding sites
pp. 416
Proximal Middle Cerebral Artery Occlusion Surgery for the Study of Ischemia–Reoxygenation Injury in the Brain
pp. 418
[24] Inferring phylogenies from protein sequences by parsimony, distance, and likelihood methods
pp. 419
[28] Enzyme immunoassay ELISA and EMIT
pp. 422
[73] α-Putrescinylthymine
pp. 425
[33] One-dimensional gel electrophoresis
pp. 425
[18] Low frequency vibrations and the dynamics of proteins and polypeptides
pp. 425
[28] Homologies and family relationships among Na+/Cl− neurotransmitter transporters
pp. 430
Colorimetric assay for chitinase
pp. 431
[41] Modification of histidyl residues in proteins by diethylpyrocarbonate
pp. 432
[47] Oligonucleotide probes for the screening of recombinant DNA libraries
pp. 434
[40] Electrophoretic analysis of chloroplast proteins
pp. 436
[41] Identification of atrial natriuretic peptide receptors in cultured cells
pp. 437
[17] The kinetics of reversible tight-binding inhibition
pp. 441
[78] Formation of rhamnolipids of Pseudomonas aeruginosa
pp. 441
[53] Lipoxygenase from soybeans
pp. 441
[30] Gel protein stains: Silver stain
pp. 443
[26] Detection of O-Linked N-acetylglucosamine (O-GlcNAc) on cytoplasmic and nuclear proteins
pp. 447
[73] Spectrophotometric and turbidimetric methods for measuring proteins
pp. 447
[39] Transformation of filamentous fungi based on hygromycin b and phleomycin resistance markers
pp. 452
[42] Analytical procedures for the isolation, identification, estimation, and investigation of the chlorophylls
pp. 455
[25] Sequencing of peptides by tandem mass spectrometry and high-energy collision-induced dissociation
pp. 458
[51] Gene isolation by screening λgt11 libraries with antibodies
pp. 459
[32] Production and properties of chimeric antibody molecules
pp. 459
[35] Isoelectric focusing
pp. 460
[27] Local alignment statistics
pp. 461
[29] Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli
pp. 463
[73] Determination of nonheme iron, total iron, and copper
pp. 468
[32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors
pp. 469
[50] Rapid isolation of lipoproteins and assessment of their peroxidation by high-performance liquid chromatography postcolumn chemiluminescence
pp. 472
Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein
pp. 476
[16] Rubber transferase from Hevea brasiliensis latex
pp. 477
[36] Gel-staining techniques
pp. 478
[39] Preparation of modified tubulins
pp. 479
[18] Voltammetric studies of redox-active centers in metalloproteins adsorbed on electrodes
pp. 480
[42] Radiolabeling of the sphingolipid backbone
pp. 481
[33] Structural analysis of RNA using chemical and enzymatic probing monitored by primer extension
pp. 483
[36] Optimizing the biolistic process for different biological applications
pp. 484
[59] Glutathione reductase
pp. 484
[42]Assessment of mitochondrial oxidative phosphorylation in patient muscle biopsies, lymphoblasts, and transmitochondrial cell lines
pp. 489
[39] Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells
pp. 490
[60] Glutathione peroxidase
pp. 494
[28] Phase determination from multiwavelength anomalous diffraction measurements
pp. 497
[34] Expression of functional antibody Fv and Fab fragments in Escherichia coli
pp. 499
[38] Purification of membrane proteins
pp. 500
[86] Levansucrase from Bacillus subtilis
pp. 504
[28] In vivo incorporation of unnatural amino acids into ion channels in Xenopus oocyte expression system
pp. 513
[30] Prediction and analysis of coiled-coil structures
pp. 515
[88] Enzymes of glycogen debranching: Amylo-1,6-glucosidase (I) and oligo-1,4→1,4-glucantransferase (II)
pp. 516
[66] Cysteine S-conjugate N-acetyltransferase
pp. 516
Osteocalcin: Isolation, characterization, and detection
pp. 518
[32] Metabolite levels in specific cells and subcellular compartments of plant leaves
pp. 523
[29] Preparation of selenomethionyl proteins for phase determination
pp. 524
[47] Establishing homologies in protein sequences
pp. 525
[89] Enzymes of glycogen degradation in biopsy material
pp. 525
[31] PHD: Predicting one-dimensional protein structure by profile-based neural networks
pp. 528
[54] Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles
pp. 534
[57] Inorganic carbon uptake by cyanobacteria
pp. 535
[41] Cathepsin B, cathepsin H, and cathepsin L
pp. 538
[40] Localized protonic coupling: Overview and critical evaluation of techniques
pp. 540
[32] GOR method for predicting protein secondary structure from amino acid sequence
pp. 546
Molecular cloning of invasion genes from Yersinia and Salmonella
pp. 548
[70] Determination of glutathione and glutathione disulfide in biological samples
pp. 554
[33] Analysis of compositionally biased regions in sequence databases
pp. 555
[95] Pullulanase (an amylopectin and glycogen debranching enzyme) from Aerobacter aerogenes
pp. 556
[58] Direct sequencing of denatured plasmid DNA
pp. 563
[45] Zymographic techniques for detection and characterization of microbial proteases
pp. 565
[92] The preparation and properties of microsomal TPNH-cytochrome c reductase from pig liver
pp. 569
[25] Monte Carlo simulations of membranes: Phase transition of small unilamellar dipalmitoylphosphatidylcholine vesicles
pp. 571
[98] Mammalian glycosidases and their inhibition by aldonolactones
pp. 574
[92a] Microsomal lipid peroxidation
pp. 575
[34] GATEWAY recombinational cloning: Application to the cloning of large numbers of open reading frames or ORFeomes
pp. 579
[56] Determination of contents and redox states of ubiquinone and menaquinone
pp. 581
[40] Use of retroviral vectors for gene transfer and expression
pp. 602
[45] Limited N-terminal sequence analysis
pp. 603
[103] A cellulolytic enzyme from Penicillium notatum
pp. 604
[65] Cosmid vectors for genomic walking and rapid restriction mapping
pp. 607
[45] Transport function and substrate specificity of multidrug resistance protein
pp. 611
[361 Construction of cDNA libraries from single cells
pp. 612
[28] Multiple antigen peptide system
pp. 613
[69] Overview of metallothionein
pp. 615
[105] A bacterial dextranase
pp. 618
[42] Receptor-mediated transport of DNA into eukaryotic cells
pp. 626
[39] Unified approach to alignment and phylogenies
pp. 628
[107] Pectin lyase from fungi
pp. 632
[108] Exopolygalacturonate lyase from Clostridium multifermentans
pp. 632
[43] Measurement of glial transport currents in microcultures: Application to excitatory neurotransmission
pp. 636
[109] α-1,4-Polygalacturonide glycanohydrolase (endo-polygalacturonase) from Saccharomyces fragilis
pp. 637
[47] Quantifying effects of antifouling paints on microbial biofilm formation
pp. 642
[67] Separation of the inner (cytoplasmic) and outer membranes of gram-negative bacteria
pp. 644
[111] Chitinases
pp. 650
[112] Bacterial mucopolysaccharidases (mucopolysaccharide lyases)
pp. 655
[47] Caveolae purification and glycosylphosphatidylinositol-linked protein sorting in polarized epithelia
pp. 667
[69] Isolation of the cell membrane of Halobacterium halobium and its fractionation into red and purple membrane
pp. 675
[27] Circular dichroism and optical rotatory dispersion of proteins and polypeptides
pp. 677
[116] Neuraminidase (sialidase) from influenza virus
pp. 680
[117] Sialidase from Clostridium perfringens
pp. 680
[99] Determination of nucleic acids in tissues by pentose analysis
pp. 684
[71] Use of eukaryotic expression technology in the functional analysis of cloned genes
pp. 685
[118] Enzymes that degrade bacterial cell walls
pp. 703
[109] Methods for the elevation of hepatic microsomal mixed function oxidase levels and cytochrome P-450
pp. 704
[51] Genetic analysis using random amplified polymorphic DNA markers
pp. 726
[100] A procedure for the isolation of deoxyribonucleic acid from microorganisms
pp. 734
[63] Properties of detergents
pp. 737
[57] Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria
pp. 737
[59] Inhibitors of lysosomal function
pp. 747
[83] Conjugal transfer of DNA to cyanobacteria
pp. 766
[85] Construction of specific mutations in photosystem II photosynthetic reaction center by genetic engineering methods in Synechocystis 6803
pp. 796
[44] Analysis of RNA hydrolyzates by liquid chromatography-mass spectrometry
pp. 805
[49] DNA analysis in the diagnosis of hemoglobin disorders
pp. 819
[117] Chromatographic determination of amino acids by the use of automatic recording equipment
pp. 855
[51] Gene targeting in embryonic stem cells
pp. 942
[36] Automated edman degradation: The protein sequenator
pp. 981
[144] Determination of nitrate and nitrite
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