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      Modern Synthetic Methods 1989 

      Biotransformations in Organic Synthesis

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      Springer Berlin Heidelberg

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          Quantitative analyses of biochemical kinetic resolutions of enantiomers

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            Enzymatic catalysis in organic media at 100 degrees C.

            Porcine pancreatic lipase catalyzes the transesterification reaction between tributyrin and various primary and secondary alcohols in a 99 percent organic medium. Upon further dehydration, the enzyme becomes extremely thermostable. Not only can the dry lipase withstand heating at 100 degrees C for many hours, but it exhibits a high catalytic activity at that temperature. Reduction in water content also alters the substrate specificity of the lipase: in contrast to its wet counterpart, the dry enzyme does not react with bulky tertiary alcohols.
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              Enzyme-catalyzed processes in organic solvents.

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                Author and book information

                Book Chapter
                1989
                : 1-114
                10.1007/978-3-642-83758-6_1
                6322f2c6-570b-42c5-9757-1aeb93672832
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