Colorimetric and Fluorimetric Detection of Amino Acids

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Author(s): G. A. Rosenthal

Publication date (Print): 1985

Publisher: Springer Netherlands

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Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.

S Udenfriend, S Stein, P Böhlen … (1972)

Fluorescamine is a new reagent for the detection of primary amines in the picomole range. Its reaction with amines is almost instantaneous at room temperature in aqueous media. The products are highly fluorescent, whereas the reagent and its degradation products are nonfluorescent. Applications are discussed.

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O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

J R Benson, P E Hare (1975)

O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.