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AIM-1: a mammalian midbody-associated protein required for cytokinesis.

Author(s): Chadwick Yasuda, M Otsu, M Tatsuka, F Suzuki, S Fujita, Y Terada

Publication date: 1998-02-02

Keywords: Amino Acid Sequence, Animals, Aurora Kinases, Cell Cycle, genetics, physiology, Cell Cycle Proteins, Cell Division, DNA, Complementary, analysis, G2 Phase, Gene Expression, Mitosis, Molecular Sequence Data, Protein Kinases, isolation & purification, Protein-Serine-Threonine Kinases, RNA, Messenger, Rats, Sequence Homology, Amino Acid, Spindle Apparatus, chemistry, Tissue Distribution

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Abstract

Mitosis is a highly coordinated process that assures the fidelity of chromosome segregation. Errors in this process result in aneuploidy which can lead to cell death or oncogenesis. In this paper we describe a putative mammalian protein kinase, AIM-1 (Aurora and Ipl1-like midbody-associated protein), related to Drosophila Aurora and Saccharomyces cerevisiae Ipl1, both of which are required for chromosome segregation. AIM-1 message and protein accumulate at G2/M phase. The protein localizes at the equator of central spindles during late anaphase and at the midbody during telophase and cytokinesis. Overexpression of kinase-inactive AIM-1 disrupts cleavage furrow formation without affecting nuclear division. Furthermore, cytokinesis frequently fails, resulting in cell polyploidy and subsequent cell death. These results strongly suggest that AIM-1 is required for proper progression of cytokinesis in mammalian cells.