Phagocytic Receptor CED-1 Initiates a Signaling Pathway for Degrading Engulfed Apoptotic Cells

Abstract

Apoptotic cells in animals are engulfed by phagocytic cells and subsequently degraded inside phagosomes. To study the mechanisms controlling the degradation of apoptotic cells, we developed time-lapse imaging protocols in developing Caenorhabditis elegans embryos and established the temporal order of multiple events during engulfment and phagosome maturation. These include sequential enrichment on phagocytic membranes of phagocytic receptor cell death abnormal 1 (CED-1), large GTPase dynamin (DYN-1), phosphatidylinositol 3-phosphate (PI(3)P), and the small GTPase RAB-7, as well as the incorporation of endosomes and lysosomes to phagosomes. Two parallel genetic pathways are known to control the engulfment of apoptotic cells in C. elegans. We found that null mutations in each pathway not only delay or block engulfment, but also delay the degradation of engulfed apoptotic cells. One of the pathways, composed of CED-1, the adaptor protein CED-6, and DYN-1, controls the rate of enrichment of PI(3)P and RAB-7 on phagosomal surfaces and the formation of phagolysosomes. We further identified an essential role of RAB-7 in promoting the recruitment and fusion of lysosomes to phagosomes. We propose that RAB-7 functions as a downstream effector of the CED-1 pathway to mediate phagolysosome formation. Our work suggests that phagocytic receptors, which were thought to act specifically in initiating engulfment, also control phagosome maturation through the sequential activation of multiple effectors such as dynamin, PI(3)P, and Rab GTPases.

Author Summary

Cells undergoing programmed cell death, or apoptosis, within an animal are swiftly engulfed by phagocytes and degraded inside phagosomes, vesicles in which the apoptotic cell is bounded by the engulfing cell's membrane. Little is known about how the degradation process is triggered and controlled. We studied the degradation of apoptotic cells during the development of the nematode Caenorhabditis elegans. Aided by a newly developed live-cell imaging technique, we identified multiple cellular events occurring on phagosomal surfaces and tracked the initiation signal to CED-1, a phagocytic receptor known to recognize apoptotic cells and to initiate their engulfment. CED-1 activates DYN-1, a large GTPase, which further activates downstream events, leading intracellular organelles such as endosomes and lysosomes to deliver to phagosomes various molecules essential for the degradation of apoptotic cells. As well as establishing a temporal order of events that lead to the degradation of apoptotic cells, the results suggest that phagocytic receptors, in addition to initiating phagocytosis, promote phagosome maturation through the sequential activation of multiple effector molecules.

Abstract

The authors have identified multiple cellular events leading to the degradation of engulfed apoptotic cells in the nematode C. elegans, and found that CED-1, a phagocytic receptor thought to specifically control apoptotic-cell engulfment, activates a signaling pathway that initiates phagosome maturation.